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Provedor de dados:  ArchiMer
País:  France
Título:  A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry
Autores:  Menguy, Eva
Dumontet, Vincent
Coulombier, Noémie
Meriot, Vincent
Le Dean, Loic
Barthelemy, Vanille
Jauffrais, Thierry
Data:  2020
Ano:  2020
Palavras-chave:  Bioassay
Quantum efficiency
Non photochemical quenching
Electron transport rate
Anti algal
Resumo:  We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina). • Microalgal production at stationary phase in stirred closed photobioreactor (PBR) operating in continuous have stable photophysiological parameters, which is a prerequisite to compare the impact of different algicidal compounds. • The combination of both methods, allows to quickly assess the algicidal effect of diverse microalgal (crude) extracts on the PSII efficiency of a tested microalgae. • The method may be used to identify and isolate algicidal molecules affecting algal PSII using a bio-guided isolation protocol.
Tipo:  Text
Idioma:  Inglês

Editor:  Elsevier BV
Formato:  application/pdf
Fonte:  Methodsx (22150161) (Elsevier BV), 2020 , Vol. 7 , P. 101037 (8p.)
Direitos:  info:eu-repo/semantics/openAccess

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