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Provedor de dados:  ArchiMer
País:  France
Título:  Combining Two-Stage Testing and Interval Mapping Strategies to Detect QTL for Resistance to Bonamiosis in the European Flat Oyster Ostrea edulis
Autores:  Lallias, Delphine
Gomez-raya, L.
Haley, C. S.
Arzul, Isabelle
Heurtebise, Serge
Beaumont, A. R.
Boudry, Pierre
Lapegue, Sylvie
Data:  2009-10
Ano:  2009
Palavras-chave:  Statistical power
Survival analysis
QTL mapping
Disease resistance
Ostrea edulis
Resumo:  We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage detection strategy had a high power with a low rate of false positives and identified nine and six probable markers linked to genes of resistance and susceptibility, respectively. Parent-specific genetic linkage maps were built for the family, spanning ten linkage groups (n = 10) with an observed genome coverage of 69-84%. Three QTL were identified by interval mapping in the first parental map and two in the second. Good concordance was observed between the results obtained after the two-stage testing strategy and QTL mapping.
Tipo:  Text
Idioma:  Inglês

Editor:  Springer
Formato:  application/pdf
Fonte:  Marine Biotechnology (1436-2228) (Springer), 2009-10 , Vol. 11 , N. 5 , P. 570-584
Direitos:  2009 Springer

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