| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Insights into Dynamics of Mobile Genetic Elements in Hyperthermophilic Environments from Five New Thermococcus Plasmids
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| Autores: |
Krupovic, Mart
Gonnet, Mathieu
Ben Hania, Wajdi
Forterre, Patrick
Erauso, Gael
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| Data: |
2013-01
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| Ano: |
2013
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| Resumo: |
Mobilome of hyperthermophilic archaea dwelling in deep-sea hydrothermal vents is poorly characterized. To gain insight into genetic diversity and dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated from geographically remote hydrothermal vents. The plasmids were ascribed to two subfamilies, pTN2-like and pEXT9a-like. Gene content and phylogenetic analyses illuminated a robust connection between pTN2-like plasmids and Pyrococcus abyssi virus 1 (PAV1), with roughly half of the viral genome being composed of genes that have homologues in plasmids. Unexpectedly, pEXT9a-like plasmids were found to be closely related to the previously sequenced plasmid pMETVU01 from Methanocaldococcus vulcanius M7. Our data suggests that the latter observation is most compatible with an unprecedented horizontal transfer of a pEXT9a-like plasmid from Thermococcales to Methanococcales. Gene content analysis revealed that thermococcal plasmids encode Hfq-like proteins and toxin-antitoxin (TA) systems of two different families, VapBC and RelBE. Notably, although abundant in archaeal genomes, to our knowledge, TA and hfq-like genes have not been previously found in archaeal plasmids or viruses. Finally, the plasmids described here might prove to be useful in developing new genetic tools for hyperthermophiles.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00125/23583/21434.pdf
DOI:10.1371/journal.pone.0049044
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| Editor: |
Public Library Science
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| Relação: |
http://archimer.ifremer.fr/doc/00125/23583/
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| Formato: |
application/pdf
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| Fonte: |
Plos One (1932-6203) (Public Library Science), 2013-01 , Vol. 8 , N. 1 , P. -
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| Direitos: |
2013 Krupovic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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