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Provedor de dados:  ArchiMer
País:  France
Título:  Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila
Autores:  Gru, Cecile
Sarradin, Pierre-marie
Legoff, Hélène
Narcon, Sandrine
Caprais, Jean-claude
Lallier, Francois
Data:  1998-06
Ano:  1998
Palavras-chave:  Sulfide
Hydrothermal seawater
Riftia pachyptila
Biological fluids
Resumo:  This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to hydrothermal seawater. The organisms are distributed along a gradient of sulfide (produced by the vent) and thiosulfate. Analysis of biological fluid was performed with a new sample treatment allowing the analysis of total sulfide (free and bound to haemoglobin) with results comparable to published methods.
Tipo:  Text
Idioma:  Inglês

Editor:  Royal Soc Chemistry
Formato:  application/pdf
Fonte:  Analyst (0003-2654) (Royal Soc Chemistry), 1998-06 , Vol. 123 , N. 6 , P. 1289-1293

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