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Provedor de dados:  ArchiMer
País:  France
Título:  Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene
Autores:  Chen, M.h.
Kuo, S. T.
Renault, Tristan
Friedman, C. S.
Chang, P. H.
Data:  2012-10
Ano:  2012
Palavras-chave:  Herpes virus
Haliotis diversicolor supertexta
DNA polymerase
Polymerase chain reaction
Resumo:  A 5781-base pair (bp) fragment of genomic DNA from the Taiwanese abalone herpesvirus was obtained and showed 99% (5767/5779) homology in the nucleotide sequence and 99% (1923/1926) in the amino acid sequence with the DNA polymerase gene of the abalone herpesvirus strain Victoria/AUS/2007. Homology of the amino acid sequence with the DNA polymerase of ostreid herpesvirus 1 was 30% (563/1856). In this study, a PCR-based procedure for detecting herpesvirus infection of abalone, Haliotis diversicolor supertexta, in Taiwan was developed. The method employed primer sets targeting the viral DNA polymerase gene, and was able to amplify DNA fragments of the expected size from infected samples. Primer sets of 40f and 146r were designed for amplification of an expected PCR product of 606 bp. Combining the new PCR protocol with histopathology, this assay can serve as a reliable diagnostic for herpesvirus infections in abalone. (C) 2012 Elsevier B.V. All rights reserved.
Tipo:  Text
Idioma:  Inglês

Editor:  Elsevier Science Bv
Formato:  application/pdf
Fonte:  Journal Of Virological Methods (0166-0934) (Elsevier Science Bv), 2012-10 , Vol. 185 , N. 1 , P. 1-6
Direitos:  2012 Elsevier B.V. All rights reserved.


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