| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Primers and polymerase chain reaction conditions for DNA barcoding teleost fish based on the mitochondrial cytochrome b and nuclear rhodopsin genes
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| Autores: |
Sevilla, Rafael G.
Diez, Amalia
Noren, Michael
Mouchel, Olivier
Jerome, Marc
Verrez-bagnis, Veronique
Van Pelt, Hilde
Favre Krey, Laurence
Krey, Grigorios
Bautista, José M.
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| Data: |
2007-09
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| Ano: |
2007
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| Palavras-chave: |
Teleost
Rhodopsin
PCR primers
Mitochondrial cytochrome b
Fish
DNA barcoding
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| Resumo: |
This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2007/publication-3034.pdf
DOI:10.1111/j.1471-8286.2007.01863.x
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| Editor: |
Blackwell science
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| Relação: |
http://archimer.ifremer.fr/doc/00000/3034/
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| Formato: |
application/pdf
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| Fonte: |
Molecular Ecology Notes (1471-8278) (Blackwell science), 2007-09 , Vol. 7 , N. 5 , P. 730-734
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| Direitos: |
2007 Blackwell
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