Sabiia Seb
        Busca avançada

Botão Atualizar

Botão Atualizar

Registro completo
Provedor de dados:  ArchiMer
País:  France
Título:  In vitro effects of cadmium and mercury on Pacific oyster, Crassostrea gigas (Thunberg), haemocytes
Autores:  Gagnaire, Beatrice
Thomas Guyon, H
Renault, Tristan
Data:  2004-04
Ano:  2004
Palavras-chave:  Toxicity
Cellular activities
Flow cytometry
Crassostrea gigas
Resumo:  In the past decades. shellfish culture has developed in a significant way around the world. However, culture areas are Often Subject to recurring anthropic pollution. The recrudescent presence of industrial wastes is a source of heavy metals and results ill pollutant transfer towards the aquatic environment in estuarine areas. Because of their mode of life. bivalves. including Mussels and oysters. are suggested as ideal indicator organisms. The development of techniques allowing the analysis of the effects of Pollutants on bivalve biology may lead to the monitoring of pollutant transfer in estuarine areas. in this context. the effects of cadmium and mercury On defence mechanisms were analysed in Pacific oysters. Crassostrea gigas. Pollutant effects were tested in vitro oil oyster haemocytes. Cell viability and enzymatic activities (esterase. peroxidase. aminopeptidase, phagocytosis activities) were monitored by flow cytometry. Enzymatic phenoloxidase-like activity was also evaluated by spectrophotometry. High pollutant concentrations were used in order to detect the acute effect and to approach real Pollutant concentrations existing ill animal tissues. Cadmium induced no effect on oyster haemocytes under the tested conditions. On the contrary, mercury caused a significant haemocyte mortality after a 24 It in vitro incubation. Aminopeptidase positive cell percentage was enhanced by the pollutant, and phenoloxidase-like activity was inhibited. These in vitro results show that mercury may be expected to have tit impact oil bivalve immune functions in contaminated areas.
Tipo:  Text
Idioma:  Inglês

Editor:  Elsevier
Formato:  application/pdf
Fonte:  Fish & Shellfish Immunology (1050-4648) (Elsevier), 2004-04 , Vol. 16 , N. 4 , P. 501-512
Direitos:  2003 Elsevier Ltd. All rights reserved

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC:

Valid HTML 4.01 Transitional