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Provedor de dados:  ArchiMer
País:  France
Título:  An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs
Autores:  Pluchon, Pierre Francois
Fouqueau, Thomas
Creze, Christophe
Laurent, Sebastien
Briffotaux, Julien
Hogrel, Gaelle
Palud, Adeline
Henneke, Ghislaine
Godfroy, Anne
Hausner, Winfried
Thomm, Michael
Nicolas, Jacques
Flament, Didier
Data:  2013-11
Ano:  2013
Resumo:  In Archaea, the proteins involved in the genetic information processing pathways, including DNA replication, transcription, and translation, share strong similarities with those of eukaryotes. Characterizations of components of the eukaryotic-type replication machinery complex provided many interesting insights into DNA replication in both domains. In contrast, DNA repair processes of hyperthermophilic archaea are less well understood and very little is known about the intertwining between DNA synthesis, repair and recombination pathways. The development of genetic system in hyperthermophilic archaea is still at a modest stage hampering the use of complementary approaches of reverse genetics and biochemistry to elucidate the function of new candidate DNA repair gene. To gain insights into genomic maintenance processes in hyperthermophilic archaea, a protein-interaction network centred on informational processes of Pyrococcus abyssi was generated by affinity purification coupled with mass spectrometry. The network consists of 132 interactions linking 87 proteins. These interactions give insights into the connections of DNA replication with recombination and repair, leading to the discovery of new archaeal components and of associations between eucaryotic homologs. Although this approach did not allow us to clearly delineate new DNA pathways, it provided numerous clues towards the function of new molecular complexes with the potential to better understand genomic maintenance processes in hyperthermophilic archaea. Among others, we found new potential partners of the replication clamp and demonstrated that the single strand DNA binding protein, Replication Protein A, enhances the transcription rate, in vitro, of RNA polymerase. This interaction map provides a valuable tool to explore new aspects of genome integrity in Archaea and also potentially in Eucaryotes.
Tipo:  Text
Idioma:  Inglês

Editor:  Public Library Science
Formato:  application/pdf
Fonte:  Plos One (1932-6203) (Public Library Science), 2013-11 , Vol. 8 , N. 11 , P. -
Direitos:  2013 Pluchon et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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