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Provedor de dados:  5
País:  France
Título:  GigaTON: an extensive publicly searchable database providing a new reference transcriptome in the pacific oyster Crassostrea gigas
Autores:  Riviere, Guillaume
Klopp, Christophe
Ibouniyamine, Nabihoudine
Huvet, Arnaud
Boudry, Pierre
Favrel, Pascal
Data:  2015-12
Ano:  2015
Palavras-chave:  Oyster
Transcriptome
Development
Stress
Tissues
NGS
Resumo:  Background: The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture shellfish resources worldwide. Important efforts have been undertaken towards a better knowledge of its genome and transcriptome, which makes now C. gigas becoming a model organism among lophotrochozoans, the under-described sister clade of ecdysozoans within protostomes. These massive sequencing efforts offer the opportunity to assemble gene expression data and make such resource accessible and exploitable for the scientific community. Therefore, we undertook this assembly into an up-to-date publicly available transcriptome database: the GigaTON (Gigas TranscriptOme pipeliNe) database. Description: We assembled 2204 million sequences obtained from 114 publicly available RNA-seq libraries that were realized using all embryo-larval development stages, adult organs, different environmental stressors including heavy metals, temperature, salinity and exposure to air, which were mostly performed as part of the Crassostrea gigas genome project. This data was analyzed in silico and resulted into 56621 newly assembled contigs that were deposited into a publicly available database, the GigaTON database. This database also provides powerful and user-friendly request tools to browse and retrieve information about annotation, expression level, UTRs, splice and polymorphism, and gene ontology associated to all the contigs into each, and between all libraries. Conclusions: The GigaTON database provides a convenient, potent and versatile interface to browse, retrieve, confront and compare massive transcriptomic information in an extensive range of conditions, tissues and developmental stages in Crassostrea gigas. To our knowledge, the GigaTON database constitutes the most extensive transcriptomic database to date in marine invertebrates, thereby a new reference transcriptome in the oyster, a highly valuable resource to physiologists and evolutionary biologists.
Tipo:  Text
Idioma:  Inglês
Identificador:  https://archimer.ifremer.fr/doc/00302/41321/40520.pdf

DOI:10.1186/s12859-015-0833-4

https://archimer.ifremer.fr/doc/00302/41321/
Editor:  Biomed Central Ltd
Formato:  application/pdf
Fonte:  Bmc Bioinformatics (1471-2105) (Biomed Central Ltd), 2015-12 , Vol. 16 , N. 401 , P. 1-12
Direitos:  2015 Riviere et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommon

info:eu-repo/semantics/openAccess

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