| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigas
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| Autores: |
Rosa, Rafael D.
Alonso, Pascal
Santini, Adrien
Vergnes, Agnes
Bachere, Evelyne
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| Data: |
2015-04
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| Ano: |
2015
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| Palavras-chave: |
Mollusk bivalve
Antimicrobial peptide
Polymorphism
Gene copy number variation
High-throughput microfluidic RT-qPCR
Vibrio infection
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| Resumo: |
We report here the first evidence in an invertebrate, the oyster Crassostrea gigas, of a phenomenon of Presence-Absence Variation (PAV) affecting immune-related genes. We previously evidenced an extraordinary interindividual variability in the basal mRNA abundances of oyster immune genes including those coding for a family of antimicrobial peptides, the big defensins (Cg-BigDef). Cg-BigDef is a diverse family composed of three members: Cg-BigDef1 to -3. Here, we show that besides a high polymorphism in Cg-BigDef mRNA expression, not all individual oysters express simultaneously the three Cg-BigDefs. Moreover, in numerous individuals, no expression of Cg-BigDefs could be detected. Further investigation at the genomic level revealed that in individuals in which the transcription of one or all Cg-BigDefs was absent the corresponding Cg-bigdef gene was missing. In our experiments, no correlation was found between Cg-bigdef PAV and oyster capacity to survive Vibrio infections. The discovery of P-A immune genes in oysters leads to reconsider the role that plays the immune system in the individual adaptation to survive environmental, biotic and abiotic stresses.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00239/35009/33545.pdf
DOI:10.1016/j.dci.2014.12.002
https://archimer.ifremer.fr/doc/00239/35009/
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| Editor: |
Elsevier
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| Formato: |
application/pdf
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| Fonte: |
Developmental & Comparative Immunology (0145-305X) (Elsevier), 2015-04 , Vol. 49 , N. 2 , P. 231-238
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| Direitos: |
2014 Elsevier Ltd. All rights reserved.
info:eu-repo/semantics/openAccess
restricted use
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