| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway
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| Autores: |
Montagnani, Caroline
Avarre, Jean-christophe
De Lorgeril, Julien
Quiquand, M
Boulo, Viviane
Escoubas, Jean-michel
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| Data: |
2007
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| Ano: |
2007
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| Palavras-chave: |
Damage associated molecular pattern
Tissue inhibitor of metalloproteinase
Metalloproteinase
Mollusk immunity
Crassostrea gigas
Oyster
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| Resumo: |
In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and upstream region. A 1-kb genomic DNA fragment flanking the 5'-end of the gene contains several regulatory elements and notably three NF-kappa B binding sites. The potential involvement of these motifs in Cg-timp gene regulation is discussed. (c) 2006 Elsevier Ltd. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2007/publication-2094.pdf
DOI:10.1016/j.dci.2006.04.002
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/2094/
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| Formato: |
application/pdf
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| Fonte: |
Developmental & Comparative Immunology (0145-305X) (Elsevier), 2007 , Vol. 31 , N. 1 , P. 1-11
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| Direitos: |
2006 Elsevier
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