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Provedor de dados:  ArchiMer
País:  France
Título:  Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis
Autores:  Choquet, Gwenaelle
Soudant, P
Lambert, C
Nicolas, Jean-louis
Paillard, C
Data:  2003-12
Ano:  2003
Palavras-chave:  Cytotoxicity
Flow cytometry
Vibrio tapetis
Ruditapes philippinarum
Bivalve hemocytes
Resumo:  Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.
Tipo:  Text
Idioma:  Inglês

Editor:  Inter-research
Formato:  application/pdf
Fonte:  Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2003-12 , Vol. 57 , N. 1-2 , P. 109-116

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