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Provedor de dados:  BABT
País:  Brazil
Título:  Purification and properties of asparaginase from the testa of immature seeds of pea (Pisum sativum L.)
Autores:  Chagas,Eliana P.
Sodek,Ladaslav
Data:  2001-09-01
Ano:  2001
Palavras-chave:  Pisum sativum
Asparaginase
Purification
Properties
Resumo:  A K+-dependent asparaginase (E.C. 3.5.1.1.) was purified 1328-fold from the testas of immature pea seeds (Pisum sativum L., var. Bolero) and characterized. Antibodies raised against purified asparaginase cross-reacted with the putative asparaginase band in Western blot analyses of semi-purified extracts. However, for crude extracts of pea testas, a cross-reaction was obtained with at least four protein bands, one of which was asparaginase protein. Affinity-purified antibodies to the four strongest bands of crude extracts were fairly specific for the bands from which they were purified, suggesting a mixture of specific antibodies. The Mr of asparaginase was 69,000 by Sephacryl S200 chromatography and also by mobility on native PAGE relative to BSA. There was no evidence for dissociation into subunits on SDS-PAGE, suggesting a monomeric protein of Mr 69,000. Other properties include an apparent Km of 2.4 mM, pI between 4.5 and 5, and competitive inhibition by aspartate and glycine.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300004
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/S1516-89132001000300004
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.44 n.3 2001
Direitos:  info:eu-repo/semantics/openAccess
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