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Provedor de dados:  BABT
País:  Brazil
Título:  Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
Autores:  Leão,Evelynne Urzêdo
Spadotti,David Marques de Almeida
Rocha,Kelly Cristina G.
Lima,Élison Fabricio B.
Data:  2017-01-01
Ano:  2017
Palavras-chave:  Molecular identification
Real-time PCR
Tospovirus vector
Resumo:  ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/1678-4324-2017160425
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.60 2017
Direitos:  info:eu-repo/semantics/openAccess

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