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Provedor de dados:  BABT
País:  Brazil
Título:  Development of an Extraction Protocol for Fetal Sexing from the Fourth Week of Gestation by Real Time PCR
Autores:  Oliveira,Edwin José Torres de
Rabacow,Ana Paula Maluf
Figueiredo,Bruno Pereira de
Ishikawa,Raíssa Borges
Pessatto,Lucas Roberto
Pelizaro,Bruno Ivo
Figueiredo,Helder Pereira de
Oliveira,Rodrigo Juliano
Data:  2019-01-01
Ano:  2019
Palavras-chave:  Fetal-sexing
DNA extraction
RtPCR.
Resumo:  Abstract Low number of fetal cells in maternal blood limited the use of fetal materials in diagnostic and clinical applications. This research developed a technology which allowed the extraction of fetal DNA by a non-invasive method that offers no risk to the mother or fetus. A total of 132 pregnant women participated in this inquiry. The DNA extraction was performed employing an in-house method based on guanidine thiocyanate and magnetic bead. For the amplification it was used the Quantifier Y Kit™. The fetal sexing analysis of the 132 pregnant women were 100% in agreement with the ultrasound. Sensitivity and specificity for detection of Y chromosome sequences was possible using Real-Time Polymerase Chain Reaction since the 4th week of gestation. This non-invasive early determination could be employed in fetal gender and also to be extended to detection of genetic diseases in the shortest possible time avoiding invasive methods that puts the fetus at risk.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100416
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/1678-4324-2019180382
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.62 2019
Direitos:  info:eu-repo/semantics/openAccess
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