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Provedor de dados:  BABT
País:  Brazil
Título:  Micropropagation of an elite F1 watermelon (Citrullus lanatus) hybrid from the shoot tip of field grown plants
Autores:  Khalekuzzaman,Mohammad
Rashid,Mohammad Harunur
Sheikh,Mominul Islam
Sharmin,Shamima Akhtar
Data:  2012-06-01
Ano:  2012
Palavras-chave:  In vitro
Hybrid vigor
Resumo:  The aim of this work was to develop a protocol for rapid micropropagation of an elite F1 hybrid watermelon cultivar using shoot tip of field-grown plants. Maximum frequency (73%) of shoot tip showed growth response in MS medium supplemented with 5 mg l-1 benzyl adenine (BA) and 0.1 mg l-1 indole-3 acetic acid (IAA). Upon transfer to cytokinin-enriched medium, the cultures produced multiple shoots and 2.0 mg l-1 BA was optimum in this respect. Addition of gibberellic acid (GA3) in the multiplication medium resulted in better growth of shoots. Rooting rate was 100% when shoots were obtained from second subculture were cultured in medium with 1.0 mg l-1 indole-3 butyric acid (IBA). The shoots produced more roots with increasing number of subcultures. About 72% of the regenerated plantlets acclimatized successfully and survived in the soil condition.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/S1516-89132012000300002
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.55 n.3 2012
Direitos:  info:eu-repo/semantics/openAccess

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