| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Damage assessment of the equine sperm membranes by fluorimetric technique
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| Autores: |
Celeghini,Eneiva Carla Carvalho
Andrade,André Furugen Cesar de
Raphael,Cláudia Fernandes
Nascimento,Juliana
Ticianelli,Janahi Souza
Arruda,Rubens Paes de
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| Data: |
2010-12-01
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| Ano: |
2010
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| Palavras-chave: |
Stallion
Acrosome
Plasma membrane
Mitochondria
Fluorescent probes
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| Resumo: |
To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this could be an efficient and practical technique to assess damage in equine spermatozoa, as it was able to determine the sperm percentage more representative of the potential to fertilize the oocyte.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000600004
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.6 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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