| Registro completo |
| Provedor de dados: |
55
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| País: |
Brazil
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| Título: |
Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil
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| Autores: |
Lancellotti,Marcelo
Pace,Fernanda de
Stehling,Eliana Guedes
Villares,Maria Cecília Barisson
Brocchi,Marcelo
Silveira,Wanderley Dias da
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| Data: |
2008-10-01
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| Ano: |
2008
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| Palavras-chave: |
Haemophilus influenzae
Characterization
Ribotyping
Serotype
Biotype
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| Resumo: |
Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2 and 9% of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and non-pathogenic strains.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500015
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| Editor: |
Brazilian Society of Infectious Diseases
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| Relação: |
10.1590/S1413-86702008000500015
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Infectious Diseases v.12 n.5 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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