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Provedor de dados:  BJID
País:  Brazil
Título:  Ribotyping, biotyping and capsular typing of Haemophilus influenzae strains isolated from patients in Campinas, southeast Brazil
Autores:  Lancellotti,Marcelo
Pace,Fernanda de
Stehling,Eliana Guedes
Villares,Maria Cecília Barisson
Silveira,Wanderley Dias da
Data:  2008-10-01
Ano:  2008
Palavras-chave:  Haemophilus influenzae
Resumo:  Forty-five Haemophilus influenzae strains isolated from patients were characterized based on biochemical characteristics. Their capsular types were determined by polymerase chain reaction (PCR); they were compared, using two molecular methods [ribotyping with a specific DNA probe amplified from the 16S rDNA region from H. influenzae and through restriction fragment length polymorphism (RLFP) of an amplified 16S DNA region]. The strains were better discriminated by the ribotyping technique that used the 16S probe and by the combination of both techniques. Biotypes I and IV were the most common, followed by biotypes VI, VIII and III. Biotypes II and VII were not found. Most of the capsular samples were nontypable (89%), with capsular types a and b found in 2 and 9% of the samples, respectively. We concluded that there is a very close genetic identity among pathogenic and non-pathogenic strains.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Brazilian Society of Infectious Diseases
Relação:  10.1590/S1413-86702008000500015
Formato:  text/html
Fonte:  Brazilian Journal of Infectious Diseases v.12 n.5 2008
Direitos:  info:eu-repo/semantics/openAccess

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