| Registro completo |
| Provedor de dados: |
BJMBR
|
| País: |
Brazil
|
| Título: |
Prolonged acceptance of skin grafts induced by B cells places regulatory T cells on the histopathology scene
|
| Autores: |
Langier,S.
Galvani,R.G.
Alves,A.P.G.
Fidelis,R.
Nunes,P.H.C.
Silva,M.H.
Castilho,L.R.
Monteiro,J.P.
Bonomo,A.
|
| Data: |
2012-10-01
|
| Ano: |
2012
|
| Palavras-chave: |
Skin graft
Tolerance
B cells
Treg
Histopathology
|
| Resumo: |
The participation of regulatory T (Treg) cells in B cell-induced T cell tolerance has been claimed in different models. In skin grafts, naive B cells were shown to induce graft tolerance. However, neither the contribution of Treg cells to B cell-induced skin tolerance nor their contribution to the histopathological diagnosis of graft acceptance has been addressed. Here, using male C57BL/6 naive B cells to tolerize female animals, we show that skin graft tolerance is dependent on CD25+ Treg cell activity and independent of B cell-derived IL-10. In fact, B cells from IL-10-deficient mice were able to induce skin graft tolerance while Treg depletion of the host inhibited 100% graft survival. We questioned how Treg cell-mediated tolerance would impact on histopathology. B cell-tolerized skin grafts showed pathological scores as high as a rejected skin from naive, non-tolerized mice due to loss of skin appendages, reduced keratinization and mononuclear cell infiltrate. However, in tolerized mice, 40% of graft infiltrating CD4+ cells were FoxP3+ Treg cells with a high Treg:Teff (effector T cell) ratio (6:1) as compared to non-tolerized mice where Tregs comprise less than 8% of total infiltrating CD4 cells with a Treg:Teff ratio below 1:1. These results render Treg cells an obligatory target for histopathological studies on tissue rejection that may help to diagnose and predict the outcome of a transplanted organ.
|
| Tipo: |
Info:eu-repo/semantics/other
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000009
|
| Editor: |
Associação Brasileira de Divulgação Científica
|
| Relação: |
10.1590/S0100-879X2012007500089
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Medical and Biological Research v.45 n.10 2012
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
