| Registro completo |
| Provedor de dados: |
BJMBR
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| País: |
Brazil
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| Título: |
Alterations in proteins of bone marrow extracellular matrix in undernourished mice
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| Autores: |
Vituri,C.L.
Alvarez-Silva,M.
Trentin,A.G.
Borelli,P.
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| Data: |
2000-08-01
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| Ano: |
2000
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| Palavras-chave: |
Fibronectin
Bone marrow
Extracellular matrix
Malnutrition
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| Resumo: |
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when compared to the control group. These results suggest an association between fluctuations in the composition of the hematopoietic microenvironment and altered hematopoiesis observed in undernourished mice.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800004
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| Editor: |
Associação Brasileira de Divulgação Científica
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| Relação: |
10.1590/S0100-879X2000000800004
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Medical and Biological Research v.33 n.8 2000
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| Direitos: |
info:eu-repo/semantics/openAccess
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