| Registro completo |
| Provedor de dados: |
BJMBR
|
| País: |
Brazil
|
| Título: |
In vitro cytotoxicity of the LDE: daunorubicin complex in acute myelogenous leukemia blast cells
|
| Autores: |
Dorlhiac-Llacer,P.E.
Marquezini,M.V.
Toffoletto,O.
Carneiro,R.C.G.
Maranhão,R.C.
Chamone,D.A.F.
|
| Data: |
2001-10-01
|
| Ano: |
2001
|
| Palavras-chave: |
Cytotoxicity
Acute myelogenous leukemia
Daunorubicin
Low-density lipoprotein
LDE complex
RT-PCR
|
| Resumo: |
Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDE:daunorubicin complex killed 46.7% of blast cells and 20.2% of normal bone marrow cells (P<0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to neoplastic cells and protecting normal tissues.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000004
|
| Editor: |
Associação Brasileira de Divulgação Científica
|
| Relação: |
10.1590/S0100-879X2001001000004
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Medical and Biological Research v.34 n.10 2001
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
