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Provedor de dados:  BJMBR
País:  Brazil
Título:  Phenotyping and genotyping methods applied to investigate the relatedness of Brazilian isolates of Enterobacter cloacae
Autores:  Darini,A.L.C.
Data:  1999-09-01
Ano:  1999
Palavras-chave:  Enterobacter cloacae
Pulsed-field gel electrophoresis
Nosocomial infection
Resumo:  In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups depending on the method used, and consequently were not reliable. Ribotyping and PFGE were significantly correlated with the clinical epidemiological analysis. PFGE did not type strains containing nonspecific DNase. Ribotyping was the most discriminative method for typing Brazilian isolates of E. cloacae.
Tipo:  Info:eu-repo/semantics/other
Idioma:  Inglês
Editor:  Associação Brasileira de Divulgação Científica
Relação:  10.1590/S0100-879X1999000900004
Formato:  text/html
Fonte:  Brazilian Journal of Medical and Biological Research v.32 n.9 1999
Direitos:  info:eu-repo/semantics/openAccess

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