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Provedor de dados:  BJMBR
País:  Brazil
Título:  A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus
Autores:  Cardoso,T.C.
Mouro-Sousa,R.L.
Oliveira,C.
Stringhini,G.
Augusto-Pinto,A.
Data:  1999-06-01
Ano:  1999
Palavras-chave:  Liquid phase blocking ELISA
Humoral response
Infectious bronchitis virus
Resumo:  A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88%), specificity (100%) and agreement (95.31%).
Tipo:  Info:eu-repo/semantics/other
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600010
Editor:  Associação Brasileira de Divulgação Científica
Relação:  10.1590/S0100-879X1999000600010
Formato:  text/html
Fonte:  Brazilian Journal of Medical and Biological Research v.32 n.6 1999
Direitos:  info:eu-repo/semantics/openAccess
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