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Provedor de dados:  BJMBR
País:  Brazil
Título:  Searching for the best real-time RT-PCRs to detect Zika virus infections: the importance of comparing several protocols
Autores:  de Moraes,F.M.
Espósito,D.L.A.
Klein,T.M.
da Fonseca,B.A.L.
Data:  2018-01-01
Ano:  2018
Palavras-chave:  Zika virus
Flavivirus
Real time RT-PCR
Diagnosis
Resumo:  Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika diagnosis is best achieved by real-time RT-PCR. However, some real-time RT-PCR show better performance than others. To reach the best possible Zika diagnosis, the analytic sensitivity of some probe-based real-time RT-PCR amplifying Zika virus RNA was evaluated in spiked and clinical samples. We evaluated primers and probes to detect Zika virus, which had been published before, and tested sensitivity using serum spiked and patient samples by real-time RT-PCR. When tested against spiked samples, the previously described primers showed different sensitivity, with very similar results when samples from patients (serum and urine) were analyzed. Real-time RT-PCR designed to amplify Zika virus NS1 showed the best analytical sensitivity for all samples.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600618
Editor:  Associação Brasileira de Divulgação Científica
Relação:  10.1590/1414-431x20187221
Formato:  text/html
Fonte:  Brazilian Journal of Medical and Biological Research v.51 n.6 2018
Direitos:  info:eu-repo/semantics/openAccess
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