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Provedor de dados:  BJM
País:  Brazil
Título:  Determination of lytic enzyme activities of indigenous Trichoderma isolates from Pakistan
Autores:  Asad,Saeed Ahmad
Hassan,Fayyaz ul
Khan,Sabaz Ali
Data:  2015-12-01
Ano:  2015
Palavras-chave:  Lytic enzymes
Mycoparasitic activity
Rhizoctonia solani
Resumo:  Abstract This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (DNS) method using suitable substrates. The antagonists were cultured in minimal salt medium with the following modifications: medium A (1 g of glucose), medium B (0.5 g of glucose + 0.5 g of deactivated R. solani mycelia), medium C (1.0 g of deactivated respective antagonist mycelium) and medium D (1 g of deactivated R. solani mycelia). T asperellum showed presence of higher amounts of chitinases, β-1, 3-glucanases and xylanases in extracellular protein extracts from medium D as compared to medium A. While, the higher activities of glucosidases and endoglucanses were shown in medium D extracts by T. harzianum. β-glucosidase activities were lower compared with other enzymes; however, activities of the extracts of medium D were significantly different. T. asperellum exhibited maximum inhibition (97.7%). On the other hand, Trichoderma sp. did not show any effect on mycelia growth of R. solani on crude extract.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-838246420140787
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.46 n.4 2015
Direitos:  info:eu-repo/semantics/openAccess

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