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Provedor de dados:  BJM
País:  Brazil
Título:  'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation
Autores:  Smaniotto,Alessandra
Skovronski,Aline
Rigo,Elisandra
Tsai,Siu Mui
Durrer,Ademir
Foltran,Lillian Liva
Di Luccio,Marco
Oliveira,J. Vladimir
Oliveira,Débora de
Treichel,Helen
Data:  2012-12-01
Ano:  2012
Palavras-chave:  Sporidiobolus pararoseus
Experimental design
'synthetic lipase'
Submerged fermentation
Resumo:  The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (2(4-1)), yeast extract and NaCl concentrations were tested to further optimization by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold increase compared to the initial medium. The time course of 'synthetic lipase' production in the optimized condition was evaluated in terms of synthetic activity, protease activity, biomass and total carbon and the maximum synthetic activity was observed during the stationary phase of growth.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400033
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822012000400033
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.43 n.4 2012
Direitos:  info:eu-repo/semantics/openAccess
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