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Provedor de dados:  BJM
País:  Brazil
Título:  LAMP technology: Rapid identification of Brucella and Mycobacterium avium subsp. paratuberculosis
Autores:  Trangoni,Marcos D.
Gioffré,Andrea K.
Cerón Cucchi,María E.
Caimi,Karina C.
Zumárraga,Martín J.
Cravero,Silvio L.
Data:  2015-06-01
Ano:  2015
Palavras-chave:  Loop-mediated isothermal amplification
Molecular typing
Resumo:  In this study, we developed new sets of primers to detect Brucella spp. and M. avium subsp. paratuberculosis (MAP) through isothermal amplification. We selected a previously well-characterized target gene, bscp31, specific for Brucella spp. and IS900 for MAP. The limits of detection using the loop-mediated isothermal amplification (LAMP) protocols described herein were similar to those of conventional PCR targeting the same sequences. Hydroxynaphtol blue and SYBR GreenTM allowed direct naked-eye detection with identical sensitivity as agarose gel electrophoresis. We included the LAMP-based protocol in a rapid identification scheme of the respective pathogens, and all tested isolates were correctly identified within 2 to 3 h. In addition, both protocols were suitable for specifically identifying the respective pathogens; in the case of Brucella, it also allowed the identification of all the biovars tested. We conclude that LAMP is a suitable rapid molecular typing tool that could help to shorten the time required to identify insidious bacteria in low-complexity laboratories, mainly in developing countries.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-838246220131206
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.46 n.2 2015
Direitos:  info:eu-repo/semantics/openAccess

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