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Provedor de dados:  BJM
País:  Brazil
Título:  Enterotoxigenic and non-enterotoxigenic Bacteroides fragilis from fecal microbiota of children
Autores:  Ignacio,Aline
Fernandes,Miriam Rodriguez
Avila-Campos,Mario Julio
Nakano,Viviane
Data:  2015-12-01
Ano:  2015
Palavras-chave:  Bacteroides fragilis
Children
ETBF
NTBF
Resumo:  Enterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II and III, based on the absence or presence of a BfPAI-flanking region, respectively. The incidence of ETBF and NTBF strains in fecal samples isolated from children without acute diarrhea or any other intestinal disorders was determined. All 84 fecal samples evaluated were B. fragilis-positive by PCR, four of them harbored the bft gene, 27 contained the NTBF pattern III DNA sequence, and 52 were considered to be NTBF pattern II samples. One sample was positive for both ETBF and NTBF pattern III DNA sequences. All 19 B. fragilis strains isolated by the culture method were bft-negative, 9 belonged to pattern III and 10 to pattern II. We present an updated overview of the ETBF and NTBF incidence in the fecal microbiota of children from Sao Paulo City, Brazil.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401141
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-838246420140728
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.46 n.4 2015
Direitos:  info:eu-repo/semantics/openAccess
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