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Provedor de dados:  BJM
País:  Brazil
Título:  PCR detection of four periodontopathogens from subgingival clinical samples
Autores:  Avila-Campos,Mario Julio
Data:  2003-04-01
Ano:  2003
Palavras-chave:  Putative periodontal pathogens
PCR
Periodontal disease
Resumo:  In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (1:10), DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly associated with disease showing a higher OR values for B. forsythus (2.97, 95% CI 1.88 - 4.70). These results suggest a strong association among the studied species and the periodontal lesion.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100017
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822003000100017
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.34 n.1 2003
Direitos:  info:eu-repo/semantics/openAccess
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