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Provedor de dados:  BJM
País:  Brazil
Título:  Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain
Autores:  Smaniotto,Alessandra
Skovronski,Aline
Rigo,Elisandra
Tsai,Siu Mui
Durrer,Ademir
Foltran,Lillian Liva
Paroul,Natália
Luccio,Marco Di
Oliveira,J. Vladimir
Oliveira,Débora de
Treichel,Helen
Data:  2014-01-01
Ano:  2014
Palavras-chave:  Sporidiobolus pararoseus
'synthetic lipase'
Submerged fermentation
Enzyme characterization
Resumo:  Lipases produced by a newly isolated Sporidiobolus pararoseus strain have potential catalytic ability for esterification reactions. After production, the enzymatic extracts (conventional crude and precipitated, 'CC' and 'CP', and industrial crude and precipitated, 'IC' e 'IP') were partially characterized. The enzymes presented, in general, higher specificity for short chain alcohols and fatty acids. The precipitated extract showed a good thermal stability, higher than that for crude enzymatic extracts. The 'CC' and 'CP' enzymes presented high activities after exposure to pH 6.5 and 40 ºC. On the other hand, the 'IC' and 'IP' extracts kept their activities in a wide range of pH memory but presented preference for higher reaction temperatures. Preliminary studies of application of the crude lipase extract in the enzymatic production of geranyl propionate using geraniol and propionic acid as substrates in solvent-free system led to a reaction conversion of 42 ± 1.5%.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100043
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822014000100043
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.45 n.1 2014
Direitos:  info:eu-repo/semantics/openAccess
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