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Provedor de dados:  BJM
País:  Brazil
Título:  Phylogenetic analysis of Dengue virus 1 isolated from South Minas Gerais, Brazil
Autores:  Drumond,Betania Paiva
Fagundes,Luiz Gustavo da Silva
Rocha,Raissa Prado
Fumagalli,Marcilio Jorge
Araki,Carlos Shigueru
Colombo,Tatiana Elisa
Nogueira,Mauricio Lacerda
Castilho,Thiago Elias
Silveira,Nelson José Freitas da
Malaquias,Luiz Cosme Cotta
Coelho,Luiz Felipe Leomil
Data:  2016-03-01
Ano:  2016
Palavras-chave:  Dengue virus-1
Alfenas
Minas Gerais
Phylogenetic analysis
Resumo:  Abstract Dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. Primary infection with a single Dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease, called dengue hemorrhagic fever. The four Dengue virus serotypes (1–4) are antigenically and genetically distinct and each serotype is composed of multiple genotypes. In this study we isolated one Dengue virus 1 serotype, named BR/Alfenas/2012, from a patient with dengue hemorrhagic fever in Alfenas, South Minas Gerais, Brazil and molecular identification was performed based on the analysis of NS5 gene. Swiss mice were infected with this isolate to verify its potential to induce histopathological alterations characteristic of dengue. Liver histopathological analysis of infected animals showed the presence of inflammatory infiltrates, hepatic steatosis, as well as edema, hemorrhage and necrosis focal points. Phylogenetic and evolutionary analyses based on the envelope gene provided evidence that the isolate BR/Alfenas/2012 belongs to genotype V, lineage I and it is probably derived from isolates of Rio de Janeiro, Brazil. The isolate BR/Alfenas/2012 showed two unique amino acids substitutions (SER222THRE and PHE306SER) when compared to other Brazilian isolates from the same genotype/lineage. Molecular models were generated for the envelope protein indicating that the amino acid alteration PHE 306 SER could contribute to a different folding in this region located within the domain III. Further genetic and animal model studies using BR/Alfenas/2012 and other isolates belonging to the same lineage/genotype could help determine the relation of these genetic alterations and dengue hemorrhagic fever in a susceptible population.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100251
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1016/j.bjm.2015.11.016
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.47 n.1 2016
Direitos:  info:eu-repo/semantics/openAccess
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