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Provedor de dados:  BJM
País:  Brazil
Título:  LEUKOTOXIC ACTIVITY OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS ISOLATED FROM HUMAN AND NON-HUMAN PRIMATES
Autores:  Lima,Francisca Lúcia de
Farias,Flávio Furtado de
Campos,Patrícia Cota
Totola,Antônio Helvécio
Tavares,Carlos Alberto Pereira
Costa,José Eustáquio da
Farias,Luiz de Mácêdo
Carvalho,Maria Auxiliadora Roque de
Data:  2001-10-01
Ano:  2001
Palavras-chave:  Actinobacillus actinomycetemcomitans
Periodontopathogen
Leukotoxic activity
Chemiluminescence assay
Resumo:  Actinobacillus actinomycetemcomitans is a clinically relevant periodontopathogenic Gram-negative coccobacillus that produces a leukotoxin of the RTX cytolysin family. In this study, we evaluated the leukotoxic activity of A. actinomycetemcomitans strains isolated from human and marmosets by Trypan blue exclusion and by the chemiluminescence assays. Among eight A. actinomycetemcomitans human strains studied, two (P2.17 and P8.12) were classified as high leukotoxin producers and among eight marmoset strains, one (M22.11) showed high leukotoxin production, as determined by Trypan blue exclusion assay. The reference strains ATCC 29523 and FDC Y4 respectively behaved like moderate and low producers. The chemiluminescence assay was used to evaluate the leukotoxic activity of M22.11 and P2.17 strains submitted to different growth conditions. Leukotoxic activity was detected on cells at the logarithmic phase and was similar under anaerobic and microaerophilic growth conditions. It was greatly reduced when cells were grown at glucose concentrations lower or higher than 0.75% (0.25% and 1.5%) in thioglycolate medium. Leukotoxin production mainly by the M22.11 strain was low in BHI broth, whereas production in TSB medium showed a similar level as in thioglycolate broth medium. Sodium bicarbonate at 10 mM did not affect leukotoxin production.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300018
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822001000300018
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.32 n.3 2001
Direitos:  info:eu-repo/semantics/openAccess
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