| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China
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| Autores: |
Li,Jing
Hu,Zhidong
Hu,Qiaojuan
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| Data: |
2012-09-01
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| Ano: |
2012
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| Palavras-chave: |
Klebsiella pneumoniae，metallo-β-lactamases
Integron
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| Resumo: |
This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin medical university general hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. ClassⅠintegron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in ClassⅠintegron 5'CS. The gene determinants were organized in the order of blaIMP-4-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300010
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822012000300010
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.43 n.3 2012
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| Direitos: |
info:eu-repo/semantics/openAccess
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