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Provedor de dados:  BJM
País:  Brazil
Título:  Isolation and characterization of an Ashbya gossypii mutant for improved riboflavin production
Autores:  Wei,Shiping
Hurley,James
Jiang,Zhenglong
Wang,Siwen
Wang,Yuanyuan
Data:  2012-06-01
Ano:  2012
Palavras-chave:  Riboflavin
Ashbya gossypii
Ultraviolet irradiation
Medium optimization
Resumo:  The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin production through dynamic analysis of its growth phase parameters, and riboflavin production could reach 8.12 g/l with pH was adjusted to the range of 6.0-7.0 using KH2PO4 in the later growth phase. This mutant has the potential to be used for industrial scale riboflavin production.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200003
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822012000200003
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.43 n.2 2012
Direitos:  info:eu-repo/semantics/openAccess
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