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Provedor de dados:  BJM
País:  Brazil
Título:  NaCl stress impact on the key enzymes in glycolysis from Lactobacillus bulgaricus during freeze-drying
Autores:  Li,Chun
Sun,Jinwei
Qi,Xiaoxi
Liu,Libo
Data:  2015-12-01
Ano:  2015
Palavras-chave:  Lactobacillus bulgaricus
Freeze-drying
NaCl stress
Semi-quantitative RT-PCR
Glycolysis
Resumo:  Abstract The viability of Lactobacillus bulgaricus in freeze-drying is of significant commercial interest to dairy industries. In the study, L.bulgaricus demonstrated a significantly improved (p < 0.05) survival rate during freeze-drying when subjected to a pre-stressed period under the conditions of 2% (w/v) NaCl for 2 h in the late growth phase. The main energy source for the life activity of lactic acid bacteria is related to the glycolytic pathway. To investigate the phenomenon of this stress-related viability improvement in L. bulgaricus, the activities and corresponding genes of key enzymes in glycolysis during 2% NaCl stress were studied. NaCl stress significantly enhanced (p < 0.05) glucose utilization. The activities of glycolytic enzymes (phosphofructokinase, pyruvate kinase, and lactate dehydrogenase) decreased during freeze-drying, and NaCl stress were found to improve activities of these enzymes before and after freeze-drying. However, a transcriptional analysis of the corresponding genes suggested that the effect of NaCl stress on the expression of the pfk2 gene was not obvious. The increased survival of freeze-dried cells of L. bulgaricus under NaCl stress might be due to changes in only the activity or translation level of these enzymes in different environmental conditions but have no relation to their mRNA transcription level.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401193
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-838246420140595
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.46 n.4 2015
Direitos:  info:eu-repo/semantics/openAccess
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