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Provedor de dados:  60
País:  Brazil
Título:  A simple HPLC method for the determination of halcinonide in lipid nanoparticles: development, validation, encapsulation efficiency, and in vitro drug permeation
Autores:  Lopes,Clarissa Elize
Langoski,Gisele
Klein,Traudi
Ferrari,Priscileila Colerato
Farago,Paulo Vitor
Data:  2017-01-01
Ano:  2017
Palavras-chave:  Halcinonide/encapsulation efficiency
Halcinonide/quantification
Polymeric lipid-core nanoparticles
Solid lipid nanoparticles
Topical administration
Toxicity
High performance liquid chromatography/method validation
Resumo:  ABSTRACT Halcinonide is a high-potency topical glucocorticoid used for skin inflammation treatments that presents toxic systemic effects. A simple and quick analytical method to quantify the amount of halcinonide encapsulated into lipid nanoparticles, such as polymeric lipid-core nanoparticles and solid lipid nanoparticles, was developed and validated regarding the drug's encapsulation efficiency and in vitro permeation. The development and validation of the analytical method were carried out using the high performance liquid chromatography with the UV detection at 239 nm. The validation parameters were specificity, linearity, precision and accuracy, limits of detection and quantitation, and robustness. The method presented an isocratic flow rate of 1.0 mL.min-1, a mobile phase methanol:water (85:15 v/v), and a retention time of 4.21 min. The method was validated according to international and national regulations. The halcinonide encapsulation efficiency in nanoparticles was greater than 99% and the in vitro drug permeation study showed that less than 9% of the drug permeated through the membrane, indicating a nanoparticle reservoir effect, which can reduce the halcinonide's toxic systemic effects. These studies demonstrated the applicability of the developed and validated analytical method to quantify halcinonide in lipid nanoparticles.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200604
Editor:  Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação:  10.1590/s2175-97902017000215250
Formato:  text/html
Fonte:  Brazilian Journal of Pharmaceutical Sciences v.53 n.2 2017
Direitos:  info:eu-repo/semantics/openAccess
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