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Provedor de dados:  BJPS
País:  Brazil
Título:  Development and validation of an analytical method using High Performance Liquid Chromatography (HPLC) to determine ethyl butylacetylaminopropionate in topical repellent formulations
Autores:  Pinto,Isadora Cabral
Freitas,Zaida Maria Faria de
Santos,Elisabete Pereira dos
Carmo,Flávia Almada do
Ricci Junior,Eduardo
Data:  2017-01-01
Ano:  2017
Palavras-chave:  Ethyl butylacetylaminopropionate (EB)/quantification
Ethyl butylacetylaminopropionate (EB)/topical formulations
Analytical method
High Performance Liquid Chromatography.
Resumo:  ABSTRACT Diseases caused by insects are frequent in poor countries, leading to epidemic scenarios in urban areas; e.g., Dengue, Zika and Chikungunya. For this reason, the development of a safe and efficient topical formulation is essential. Ethyl butylacetylaminopropionate (EB) is a mosquito repellent developed by Merck, which is used in products for adults, children and especially babies, due to its low allergenic potential. The aim of this work was to validate an analytical methodology to quantify EB in a new poloxamer-based formulation by high-performance liquid chromatography (HPLC). The quantification methodology was performed at 40 ºC using a Kromasil reverse-phase column (C18), with the dimensions of 250 x 4.6 mm. The mobile phase was acetonitrile:water (1:1) at a 1.0 mL/min flow-rate. The detector wavelength was set at 218 nm to detect EB. The methodology was considered validated since the results indicated linearity (R2>0.99), specificity, selectivity, precision and accuracy (active recovery between 98% and 102%). It also presented limits of detection and quantification of 0.255 µg/mL and 0.849 µg/mL, respectively. The present study demonstrated the EB vehiculated in poloxamer gel is promising as a new insect repellent formulation, since it could be quantified and quality control evaluated.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação:  10.1590/s2175-97902017000216033
Formato:  text/html
Fonte:  Brazilian Journal of Pharmaceutical Sciences v.53 n.2 2017
Direitos:  info:eu-repo/semantics/openAccess

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