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Provedor de dados:  60
País:  Brazil
Título:  Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil
Autores:  Oltramari,Karine
Cardoso,Rosilene Fressati
Patussi,Eliana Valéria
Santos,Adolfo Carlos Barreto
Mikcha,Jane Martha Graton
Data:  2014-06-01
Ano:  2014
Palavras-chave:  Pasteurized milk/processing
Pasteurized milk/bacterial typing
Pasteurized milk/contamination
Escherichia coli/genetic diversity
Escherichia coli/antimicrobial resistance
Food/microbiological analysis
Resumo:  Food contamination caused by enteric pathogens is a major cause of diarrheal disease worldwide, resulting in high morbidity and mortality and significant economic losses. Bacteria are important agents of foodborne diseases, particularly diarrheagenic Escherichia coli. The present study assessed the genetic diversity and antimicrobial resistance of E. coli isolates from pasteurized milk processed in 21 dairies in northwestern State of Parana, Brazil. The 95 E. coli isolates were subjected to antimicrobial susceptibility testing according to the recommendations of the Clinical and Laboratory Standards Institute and assessed genotypically by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The highest rate of resistance was observed for cephalothin (55.78%). ERIC-PCR revealed high genetic diversity, clustering the 95 bacterial isolates into 90 different genotypic patterns. These results showed a heterogeneous population of E. coli in milk samples produced in the northwestern region of Paraná and the need for good manufacturing practices throughout the processing of pasteurized milk to reduce the risk of foodborne illnesses.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação:  10.1590/S1984-82502014000200013
Formato:  text/html
Fonte:  Brazilian Journal of Pharmaceutical Sciences v.50 n.2 2014
Direitos:  info:eu-repo/semantics/openAccess

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