| Registro completo |
| Provedor de dados: |
60
|
| País: |
Brazil
|
| Título: |
Deregulated expression of A1, Bcl-2, Bcl-xL, and Mcl-1 antiapoptotic proteins and Bid, Bad, and Bax proapoptotic genes in polycythemia vera patients
|
| Autores: |
Gasparotto,Elainy Patricia Lino
Tognon,Raquel
Ferreira,Aline Fernanda
Oliveira,Gislane Lelis Vilela
Palma,Patrícia Vianna Bonini
Zanichelli,Maria Aparecida
Souto,Elizabeth Xisto
Velano,Carlos Eduardo Engel
Simões,Belinda Pinto
Carrara,Rita de Cassia Viu
Kashima,Simone
Covas,Dimas Tadeu
Castro,Fabíola Attie de
Souza,Ana Maria de
|
| Data: |
2011-12-01
|
| Ano: |
2011
|
| Palavras-chave: |
Polycythemia vera
Gene mutation
Gene expression
Apoptosis
Bcl-2 family members
|
| Resumo: |
Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid expression (14.4) in comparison with healthy subjects (1.0; p=0.002). Patients' leukocytes showed an A1 augmentation (7.41, p=0.001) and a reduced expression of Bax (0.19; p=0.040) and Bad (0.2; p=0.030). There was no correlation between JAK2 V617F allele burden and molecular expression. PV patients showed alterations in Bcl-2 members' expression, which may interfere with control of apoptotic machinery and contribute to disease pathogenesis.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400025
|
| Editor: |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
|
| Relação: |
10.1590/S1984-82502011000400025
|
| Formato: |
text/html
|
| Fonte: |
Brazilian Journal of Pharmaceutical Sciences v.47 n.4 2011
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
|
