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Provedor de dados:  Braz. J. Plant Physiol.
País:  Brazil
Título:  On-line HPLC analysis of the antioxidant activity of phenolic compounds in brewed, paper-filtered coffee
Autores:  Stalmach,Angélique
Data:  2006-03-01
Ano:  2006
Palavras-chave:  Coffea
Chlorogenic acids
Resumo:  Caffeoyl-, feruloyl- and dicaffeoylquinic acids (chlorogenic acids) in infusions from green and medium roasted coffee beans were identified and quantified by reverse phase HPLC with photodiode array and MS³ detection prior to assessment of the antioxidant activity using an HPLC system with post-column on-line antioxidant detection based on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid radical scavenging activity. Caffeoylquinic acids were the most abundant antioxidants and roasting induced isomerisation with a decline in 5-O-caffeoylquinic acid and concomitant increases in the 3- and 4-O-derivatives. This did not affect the level of caffeoylquinic acid-derived antioxidant activity in the roasted coffee. Roasting did, however, result in the appearance of additional unidentified HPLC peaks with antioxidant activity. Because of this and an increase in the antioxidant activity of components that did not elute from the reversed phase HPLC column, the antioxidant capacity of the beverage derived from medium roast beans was double that of the unroasted coffee. The antioxidant activity of coffees that have undergone different degrees of roasting would, therefore, appear to be due to combinations of different components. The effect of roasting on chlorogenic acids in coffee beans is considered, and the possible contribution of Maillard reaction products to the antioxidant capacity of roasted coffees is discussed.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Brazilian Journal of Plant Physiology
Relação:  10.1590/S1677-04202006000100018
Formato:  text/html
Fonte:  Brazilian Journal of Plant Physiology v.18 n.1 2006
Direitos:  info:eu-repo/semantics/openAccess

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