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Provedor de dados:  63
País:  Brazil
Título:  Production of streptomycete inoculum in sterilized rice
Autores:  Soares,Ana Cristina Fermino
Sousa,Carla da Silva
Garrido,Marlon da Silva
Perez,Jane Oliveira
Data:  2007-12-01
Ano:  2007
Palavras-chave:  Actinomycetes
Biological control
Growth substrates
Resumo:  Actinomycetes are important plant disease control and growth promotion agents, which makes it necessary to develop technology to produce large quantities of inoculum for green-house and field work. The present study had the objective of evaluating the growth of several isolates of Streptomyces in sterile rice for inoculum production. The sterile rice was inoculated with isolates of S. thermotolerans, S. griseus subsp. griseus, Streptomyces sp. N0035, S. purpurascens, and Streptomyces sp., and incubated at 28 ± 2ºC. Five days after its inoculation, mycelial growth and sporulation was observed for all Streptomyces isolates on the rice grains. Twelve days after incubation, the colonized rice was transferred to envelopes of dark brown paper and let to dry in an incubator at 30ºC for three days. After drying, 1g of colonized rice was added to 200 mL of sterile distilled water and the number of spores was counted under a microscope with a Newbauer counting chamber. Spore production varied from 0.14 × 10(9) to 1.47 × 10(9) spores per gram of rice and differed among the Streptomyces species. Sterile rice can be an alternative substrate for low cost mass production of Streptomyces inoculum.
Tipo:  Info:eu-repo/semantics/report
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600013
Editor:  São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação:  10.1590/S0103-90162007000600013
Formato:  text/html
Fonte:  Scientia Agricola v.64 n.6 2007
Direitos:  info:eu-repo/semantics/openAccess
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