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Provedor de dados:  Scientia Agricola
País:  Brazil
Título:  In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia
Autores:  Almeida,Weliton Antonio Bastos de
Mourão Filho,Francisco de Assis Alves
Mendes,Beatriz Madalena Januzzi
Rodriguez,Adriana Pinheiro Martinelli
Data:  2002-03-01
Ano:  2002
Palavras-chave:  Adventitious bud
Genetic transformation
Resumo:  Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations. After adventitious bud growth, the shoots were transferred to MT medium with either NAA or IBA (1 mg L-1), or absence of auxin, for rooting. The best results were obtained with 1 mg L-1 BAP for bud induction and 1 mg L-1 IBA for rooting for all three sweet orange cultivars. The use of 0.5-2.5 mg L-1 BAP, followed by 1 mg L-1 IBA were the best growth regulator combinations for bud induction and rooting, respectively, for 'Rangpur' lime. The protocols presented in this work are suitable for associations with genetic transformation experiments for these cultivars.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação:  10.1590/S0103-90162002000100004
Formato:  text/html
Fonte:  Scientia Agricola v.59 n.1 2002
Direitos:  info:eu-repo/semantics/openAccess

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