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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  ITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple Glomerella leaf spot
Autores:  Giaretta,Diorvania Ribeiro
Coelho,Cileide Maria Medeiros
Guidolin,Altamir Frederico
Dantas,Adriana Cibele de Mesquita
Gomes,Eliane Aparecida
Data:  2010-04-01
Ano:  2010
Palavras-chave:  Pineapple guava
Molecular phylogeny
In silico
Phylogenetic tree
Resumo:  Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp were obtained. The sequencing of this region allowed the generation of a phylogenetic tree, regardless of their hosts, and 5 isolated groups were obtained. From the "in silico" comparison, it was possible to verify a variation from 93 to 100% of similarity between the sequences studied and the Genbank data base. The causal agent of GLS is nearly related (clustered) to isolates of pineapple guava and to the citrus isolates used as control.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/S0103-84782010005000051
Formato:  text/html
Fonte:  Ciência Rural v.40 n.4 2010
Direitos:  info:eu-repo/semantics/openAccess

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