| Registro completo |
| Provedor de dados: |
Electron. J. Biotechnol.
|
| País: |
Chile
|
| Título: |
Heat identification by 17β-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay
|
| Autores: |
Domènech,Anna
Pich,Sara
Arís,Anna
Plasencia,Carmen
Bach,Alex
Serrano,Alicia
|
| Data: |
2011-07-01
|
| Ano: |
2011
|
| Palavras-chave: |
Cattle
Estrous
Heat
Hormone
Insemination
|
| Resumo: |
Background: There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17β-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17β-estradiol pre-ovulation peak in spite of its limited concentration and short manifestation in milk. Results: Milk sample treatments for progesterone and 17β-estradiol EIA measurements are described. Hormonal profiles from daily milk samples of six different cows were reported and 17β-estradiol pre-ovulation peak was visualized in all cases. Heat detection was possible by EIA using one every 2 days milking samples in almost all studied cases. Only in one case, morning and afternoon milking samples were required to visualize the 17β-estradiol pre-ovulation peak. Conclusions: 17β-estradiol EIA quantification in raw milk is a reliable, rapid, economic and a precise method to describe cow heat along with EIA progesterone determination.
|
| Tipo: |
Journal article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400010
|
| Editor: |
Pontificia Universidad Católica de Valparaíso
|
| Formato: |
text/html
|
| Fonte: |
Electronic Journal of Biotechnology v.14 n.4 2011
|
