| Registro completo |
| Provedor de dados: |
Gayana Botánica
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| País: |
Chile
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| Título: |
Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
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| Autores: |
Coello,Christian Yanelly
Miceli,Clara Luz
Orantes,Carolina
DenDooven,Luc
Gutiérrez,Federico Antonio
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| Data: |
2010-01-01
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| Ano: |
2010
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| Palavras-chave: |
6-benzyladenine
Indole-3-acetic acid
A-naftalenacetic acid
Gibberellic acid
Shoot induction
Rooting
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| Resumo: |
An in vitro culture procedure was developed to induce shoots and roots of Guarianthe skinneri (Orchidaceae) plantlets regenerated from seed-derived protocorms on a Murashige and Skoog (MS) médium supplemented with 6-benzyladenine (BA), indole-3-acetic acid (IAA), a-naftalenacetic acid (NAA) and gibberellic acid (GA3). A máximum of 10.6 shoots was obtained with 16.1 μM NAA, 17.1 μM IAA, 6.3 x 10-9 μM GA3 and 0.0023 μM BA. A máximum of 4.0 roots on each shoot with 5.4 μM NAA, 17.1 μM IAA, 0.001 μM AG3 and 4.6 x 10-9 μM BA. Máximum shoot and root length was obtained with a minimum of GA3, but a máximum IAA. GA3, was the main factor controlling shoot and root induction and elongation.
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| Tipo: |
Journal article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432010000100003
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| Editor: |
Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
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| Formato: |
text/html
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| Fonte: |
Gayana. Botánica v.67 n.1 2010
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