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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Genetic analysis of D-xylose metabolism by endophytic yeast strains of Rhodotorula graminis and Rhodotorula mucilaginosa
Autores:  Xu,Ping
Bura,Renata
Doty,Sharon L.
Data:  2011-01-01
Ano:  2011
Palavras-chave:  Rhodotorula graminis
Rhodotorula mucilaginosa
D-xylose metabolism
Xylitol
Xylitol dehydrogenase
Xylose reductase
Resumo:  Two novel endophytic yeast strains, WP1 and PTD3, isolated from within the stems of poplar (Populus) trees, were genetically characterized with respect to their xylose metabolism genes. These two strains, belonging to the species Rhodotorula graminis and R. mucilaginosa, respectively, utilize both hexose and pentose sugars, including the common plant pentose sugar, D-xylose. The xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes were cloned and characterized. The derived amino acid sequences of xylose reductase (XR) and xylose dehydrogenase (XDH) were 32%~41% homologous to those of Pichia stipitis and Candida. spp., two species known to utilize xylose. The derived XR and XDH sequences of WP1 and PTD3 had higher homology (73% and 69% identity) with each other. WP1 and PTD3 were grown in single sugar and mixed sugar media to analyze the XYL1 and XYL2 gene regulation mechanisms. Our results revealed that for both strains, the gene expression is induced by D-xylose, and that in PTD3 the expression was not repressed by glucose in the presence of xylose.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300018
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572011000300018
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.34 n.3 2011
Direitos:  info:eu-repo/semantics/openAccess
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