Registro completo |
Provedor de dados: |
74
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País: |
Brazil
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Título: |
A low-cost apparatus for transforming Drosophila and detecting green fluorescent protein (GFP) genetic markers
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Autores: |
Deprá,Maríndia
Sepel,Lenira Maria Nunes
Loreto,Élgion Lucio da Silva
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Data: |
2004-01-01
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Ano: |
2004
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Palavras-chave: |
Transgenic Drosophila
PiggyBac
GFP
Transposable elements
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Resumo: |
We describe the transformation of white mutant Drosophila simulans with a piggyBac transposon vector and a green fluorescent marker (GFP) and show how to construct inexpensive micro-manipulation and epifluorescence equipment for use in transposon-mediated germ-line transformation. Although the number of G0 adult flies (16) obtained in relation to the number of injected eggs was very low (12.5%) it was comparable to the proportion described by other authors and can be considered as a good rate of transformation.
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Tipo: |
Info:eu-repo/semantics/article
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100012
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Editor: |
Sociedade Brasileira de Genética
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Relação: |
10.1590/S1415-47572004000100012
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Formato: |
text/html
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Fonte: |
Genetics and Molecular Biology v.27 n.1 2004
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Direitos: |
info:eu-repo/semantics/openAccess
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