| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Molecular cloning and sequence analysis of growth hormone cDNA of Neotropical freshwater fish Pacu (Piaractus mesopotamicus)
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| Autores: |
Pinheiro,Janeth Silva
Wolff,José Luiz Caldas
Araújo,Ronaldo de Carvalho
Hilsdorf,Alexandre Wagner Silva
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| Data: |
2008-01-01
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| Ano: |
2008
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| Palavras-chave: |
Piaractus mesopotamicus
Growth hormone
CDNA cloning
Sequencing
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| Resumo: |
RT-PCR was used for amplifying Piaractus mesopotamicus growth hormone (GH) cDNA obtained from mRNA extracted from pituitary cells. The amplified fragment was cloned and the complete cDNA sequence was determined. The cloned cDNA encompassed a sequence of 543 nucleotides that encoded a polypeptide of 178 amino acids corresponding to mature P. mesopotamicus GH. Comparison with other GH sequences showed a gap of 10 amino acids localized in the N terminus of the putative polypeptide of P. mesopotamicus. This same gap was also observed in other members of the family. Neighbor-joining tree analysis with GH sequences from fishes belonging to different taxonomic groups placed the P. mesopotamicus GH within the Otophysi group. To our knowledge, this is the first GH sequence of a Neotropical characiform fish deposited in GenBank.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200037
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572008000200037
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.31 n.1 suppl.0 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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