| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene
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| Autores: |
Chen,Congying
Sargent,Carole
Quilter,Claire
Yang,Zhuqing
Ren,Jun
Affara,Nabeel
Brenig,Bertram
Huang,Lusheng
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| Data: |
2010-01-01
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| Ano: |
2010
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| Palavras-chave: |
Expression profile
Molecular characterization
Physical mapping
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| Resumo: |
Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA motifs were identified in the 208-bp upstream genomic region. Five single nucleotide polymorphisms (SNPs) were detected in introns* and the 3' UTR. RT-PCR indicated that the PGRMC2 gene is expressed ubiquitously in all pig tissues examined.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300015
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572010005000057
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.33 n.3 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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